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To find antitumor constituents in Korean basidiomycetes, the mycelia of Lentinus edodes DMC7, which had shown a good mycelial growth in shakeflasks, were cultured at 27℃ on an orbital shaking incubator at 180 rev/min for 12 days. The medium was composed of glucose (50g/ℓ), yeast extract (9g/ℓ), peptone (9g/ℓ), and seven inorganic salts. A water soluble macromolecular fraction, LF-3, was obtained from the culure filtrate by fractionation with ethanol and dialysis using a Visking tube. When LF-3 was administered i.p. at 50㎎/㎏/day once daily for 10 consecutive days to female ICR mice which were implanted s.c. with sarcoma 180 (10² cells/mouse), it exerted a highly significant antitumor activity, with the tumor inhibition ratio of 53.1%.
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